How different concentration of enzyme catalase affects the rate of breaking down substrate hydrogen peroxide Essay Example

How different concentration of enzyme catalase affects the rate of breaking down substrate hydrogen peroxide Essay Example How different concentration of enzyme catalase affects the rate of breaking down substrate hydrogen peroxide Paper How different concentration of enzyme catalase affects the rate of breaking down substrate hydrogen peroxide Paper Enzymes are large proteins that speed up chemical reaction. As globular protein, enzymes have a specific three-dimensional shape which is determined by their sequence of amino acids. Despite their large overall size, enzyme molecules only have a small region that is functional. This is known as enzymes active site. The substrate molecule is held within the active site by bonds that temporarily form between the R groups of the amino acids of the active site by bonds and all groups on the substrate molecules. This structure is known as enzyme-substrate complex. Enzymes are classified into several categories, such as hydrolytic, oxidising, and educing. Depending on the types of reaction they control. In this case, the enzyme I will use in the investigation is catalase from celery extract, which is concluding as hydrolytic enzyme. This type of enzyme accelerates reactions in which a substrate is broken down into simpler compounds through reaction with adding up water molecules. Oxidising enzyme, known as oxidises, accelerate oxidation reaction; reducing enzyme speed up reducing reactions in which oxygen is removed. A substrate is the molecule, which can bind into the active of enzyme. In this case, I will use hydrogen peroxide as the substrate. Enzyme works in the same way as a key operates a lock. Enzymes active sites have a particular shape like a lock and only a particular key ( substrate) can fit into that lock. Enzymes are therefore specific in the reactions that they catalyse. This is known as the lock and key theory. In practice, unlike a rigid lock, the enzyme actually changes its form slightly to fit the shape of the substrate. In other works, it is flexible and moulds itself around the substrate. As it alters its shape, the enzyme puts a strain on the substrate molecule and thereby lowers its activation energy. In this case, enzyme catalse has a specific active site, which just for the substrate hydrogen peroxide to fit in, then the reaction takes place. The reaction involved is hydrolysis. The equation of reaction is: 2H2O2 2H2O + O2 Factor that would affect the reaction: Temperature At low temperature, the reaction takes place very slowly, this because molecules are moving relatively slowly as have low kinetic energy. Substrate molecules will not often collide with the active site, and so binding between substrate and enzyme is a rare event. Therefore, reaction is slow. A rise in temperature increases the kinetic energy of molecules which therefore move around more rapidly and collide with one another more often. This means at a higher temperature, the reaction will take place faster than a lower temperature and an increasing or a decreasing of temperature will affect my reaction and results. For this reason, I will control this effect by using a water bath to maintain the temperature the same during the whole reaction. As enzyme work best at a certain temperature, this is known as optimum temperature. If the temperature is too low, enzyme cannot work properly; if the temperature is too high, this may denature the enzyme active site and enzyme will lose its function. So to avoid this problem, I will keep the water bath has the temperature of 25?C, which is the room temperature. Therefore enzyme catalase will work properly and temperature will not be a factor to affect this investigation. PH Most enzymes also have an optimum pH at which they function best. In human body, most enzyme work fastest at an optimum pH of about 7. For example, the digestive system Pepsin found in the stomach to digest proteins. A change I pH means a change in the concentration of hydrogen ions in the surrounding of the enzyme. This affects the ionisation of R group in the amino acid residues of the protein molecule and the shape of the active site to bind with the substrate. The lower the pH, the bigger the hydrogen ions concentration it is . Hydrogen ions can interact with the R group of amino acids, affecting the way in which they bond with each other and therefore affect their 3D shape. So the lower the pH, the higher the hydrogen ions concentration it is, and therefore the slower the reaction. Oppositely, the higher the pH, the faster the reaction it will take place. To control this factor, I will keep the pH all the way through the same by using a pH paper to check the pH number during the experiment. Enzyme concentration The rate of reaction increases as the concentration of enzyme increase. Because higher concentration of enzyme means higher number of enzyme molecules, so that more enzyme molecule will collide with substrate molecule, therefore the reaction will take place faster. When there is plenty of substrate, the rate of reaction is not limited by the concentration of enzyme. Therefore if the concentration of enzyme is increased, the number of collision with the substrate molecule and hence the rate of reaction will increase. If the amount of substrate is limited, the rate of reaction decreases because if the amount of substrate is limited, the enzymes active site will not collide with substrate very often, therefore a few products will be made. As the reaction progressing, the substance will be broken down slowly, therefore less substrate left. So enzyme molecules freer, therefore products will be produced more slowly. Hence the slower the reaction goes. In this experiment, the enzyme concentration is an independent variable which I will vary the concentration of celery extract each time to investigate how different concentration of celery extract( enzyme concentration) affect the rate of breaking down hydrogen peroxide. Substrate concentration Increasing in the substrate concentration will increase in the rate of reaction. Because increasing the substrate concentration, we increased the number of substrate molecules; therefore more collision will take place between substrate and enzymes active site. Hence mere products will be produced. Therefore the faster the rate of reaction takes place. However, if we continue increase the substrate keeping the enzyme concentration and volume the same. The rate of reaction will not continue to increase. This is because a higher substrate concentration active site of all the enzyme molecule is busy with substrates. The number of enzyme molecules because a limiting factor. The rate of reaction at higher substrate becomes constant. In this case, I will keep the volume of hydrogen peroxide the same for using each time. Inhibitors Enzyme inhibitors are substances that directly or indirectly interfere with the functioning of the active of an enzyme and so reduce its activity. There are two types of inhibitors: Competitive inhibitors Competitive inhibitors are the molecules which have a shape similar to the substrate, so they fit into the active site of the enzyme. Therefore the substrate cannot enter into the active site and so the enzyme cannot catalyse the reaction. Hence, the reaction is slow down. However, the inhibitors are not permanently bound to the active site and so, when it leaves another molecule can take its place. Sooner or later, all the substrate molecules will find an active site, but the greater the concentration of inhibitors, the longer the reaction will be. Non-competitive inhibitors Non-competitive inhibitors will bind to another part of the enzyme rather than the true active site. This changes the shape of the enzymes active site so that the substrate no longer fits. Therefore the reaction is slow down. As the substrate and the inhibitor are not competry for the same site, an increase in substrate concentration does not decrease the effect of inhibitors. To control the factor of inhibitors. I will not add any substance except celery solution and hydrogen peroxide during the experiment. Prediction I predict that the higher the concentration of celery extract I use, the fast the rate of breaking down hydrogen peroxide I will get. As in higher concentration of celery extract, the more number of catalase molecules in the solution. So there are more collisions between substrate ( hydrogen peroxide) and enzyme( catalase) active site, and more products will be formed( water and oxygen). The rate of reaction can be measured by measuring the volume of oxygen produced in a period of time. The larger the volume of oxygen produced in the same period of time, the fast the reaction is taken place. Preliminary work In order to get finest and reliable results, I did a preliminary work. This enable me not just find out a suitable range of celery extract of catalase concentration I will use in my real experiment, but also help me descried the time interval I will measure the oxygen get and justify the equipment and method. I think these all lead me to get reliable and accurate results in the end. In the preliminary work, I choose to do three different concentration of celery extract. To get different concentrations of celery extract, I used distilled water to dilute the celery extract. I tested the range of celery extract between 100% 20%, the highest concentration is 100%, the lowest concentration is 20% and the median is 60%. The reason I chose these three concentrations is because between each concentration, there is 40% difference and this enable me to test that in which level of concentration, reaction goes best and to find out which concentration of celery extract I will use in my real experiment.

Discussion Board Assignment Example | Topics and Well Written Essays - 250 words - 12

Discussion Board - Assignment Example This simply means that the conditions one experiences in prisons may vary from one place to another. Therefore, asserting that convict criminologists have more credibility in regard to their information of prison life is a farfetched idea. On the other hand, prison is a dynamic place where people get convicted on a daily basis with different criminal offenses. Political systems are also changing on a daily basis meaning that new rules and regulations governing human actions are formulated and implemented each time, thus an action that was not considered a crime in the early 90’s could be considered a crime in the contemporary society and vice-versa. This creates a need for continuous learning on issues ascribed to prison life, thus one person cannot claim that owing to the fact that he was convicted ten years ago, he is in a better position to comprehend prison-related issues than other scholar. In conclusion, the credibility of convict criminologists, does matter, they have a unique source of knowledge that is not possessed by people who have never been

Violence in Education Research Paper Example | Topics and Well Written Essays - 1750 words

Violence in Education - Research Paper Example This essay highlights that  education is a very critical sector in the economy of any nation as it contributes too much in terms of expertise and professional knowledge which can be utilized in various fields for achievement of success and progress. It is also through education that many problems faced by the contemporary society are eradicated for instance poverty and ignorance. Despite its importance, education is faced with numerous challenges that hinder its success in one way or the other for example lack of adequate resources and facilities, poor management and violence among others.  According to the report findings  education is a very instrumental aspect to every individual, the society, a nation and the world at large. Every person should be entitled to education to the highest possible level with favorable conditions that allows for excellence. This is so because education is a significant building block in regard to the growth and development of human beings   as it acts as a driving force towards improved living standards and also development through aspects like reduction of poverty, promotion of peace and stability,   improvement of health, promotion of gender equality among other aspects. All this is made possible because people get enlightened when they go through various education systems which instill a lot of knowledge in a wide range of life issues hence helping them in general decision making and problem solving. Education sector is faced with a lot of challenges violence being one of them.

Pursuing Undergraduate Studies Essay Example for Free

Pursuing Undergraduate Studies Essay I lived in South Korea for 14 years with a family and friends who are always there to comfort me through thick and thin. After finishing my eighth grade my parents decided to send me in the United States of America to study.   They believed that by doing so, it will make me a stronger person both academically and socially.   At the start, it was very tough for me because of the language barrier.   My difficulties in using the English language cause a tremendous social gap with the American society.    In the long run I was able to overcome those obstacles in my life.   I have found myself enjoying my stay in the USA with new friends and a better life ahead with more open doors and opportunities.   One of these doors was when I attended a JP Art–Studio during the first year which aroused my interest in art and I accepted those changes with open arms. The only barrier of this desire is my parents, whom I don’t want to disappoint aside that they are the one supporting my study here in USA. They want me to become a doctor, but my heart and soul cried out for me to become an artist. Until one day, my life changed dramatically.   Two years ago when I visited Maryland in one of the bookstore, I saw an illustration book by Obata-Dakesi. My eyes widened, my ears numbed, my body froze and my mind got sucked as I read the illustration book of Obata-Dakisi. I heard a lot of stories of people who became an animators and illustrators after reading an illustration book. It was the turning point in my life. I cannot imagine that this kind of art has the great power to make people pick up their pen to graphically explore their world and express their emotions, as what happened to me. A strong marvelous feeling inspired me to pursue my dream of becoming an artist.   It was truly amazing how I was able to share my thoughts and feelings by just looking at other’s drawings.   It brings out the very true person as I am.   Day after day drawing has become more significant in my life.   I am fascinated with the structures of an illustration so much that it motivates and inspired me to determine strongly the purpose of my life.   I started looking at objects more closely and experimenting to it so as to build up further my drawing skills.   I become more attentive and sensitive to my surroundings. Now, I have my own definition of â€Å"art† and this makes me more comfortable following my dreams.   My goal is not becoming what everyone else wishes me to be, but to follow the desire of my heart, this burning feeling that makes me move every now and then.   I cannot imagine life without art.   It becomes my best friend, my best life-teacher.   It will make me a better unique person with purpose and direction in life.   Arts what matters to me know.   Throughout my life, I have found myself another fulfilling experience that makes me the happiest person. My ultimate goal now is to be admitted in one of the best school, the School of Visual Arts for computer art program in Maryland Institute College of Art.   Through enrolling in this prestigious institution it will be able to help me fulfill my dream in becoming an ultimate artist. I know that I will learn many techniques, styles and many more from your institution and this will all help me to prepare in my future careers in arts.

Charter Schools in Arkansas :: Teaching Education

Charter Schools in Arkansas Charter Schools Introduction charter schools have become a common site in many states today. Currently, there are over 24 states with charter schools established and many other states have passed legislation for the creation of charter schools. Arkansas passed legislation in 1996 that would allow for the creation of charter schools in the state. Governor Mike Huckabee made it a priority in his educational agenda in 1997 to allow a pilot program of 15 schools to be implemented statewide (Cohen, 1998). Even though Governor Huckabee and legislation is supporting this idea, there have been no successful applications so far. Charter applicants seek approval of the state department of education for the charter school but have meet with resistance so far. Fourche Valley School District in Yell County was the first to apply for charter school status in 1996, but was quickly turned down. The school has yet to reapply for the status. No other school districts in the state have attempted to file as of yet. Statement of the Problem. What is a charter school and what are the financial implications of creating a charter school? Review of Literature Charter schools are public schools but with a twist. They are created and operated by the educators, parents, community leaders, and others. The school must be sponsored by a designated local or state educational organization that monitors the school's progress, but that is as far as it goes. The school is free from the traditional bureaucratic red tape and policy makers from the state level. The school is basically governed at the local level. Some people view them as a local business meeting the demands of the community. The product they produce are educated children. If the quality of service is not being meet, the school answers to the community. This concept has high levels of accountability being placed on the school. As successful businesses know, if you produce a high quality product, the business will grow and succeed, if you produce anything less, you go out of business. Opening a charter school also brings with it the ability to choose the school where you want your children to go. The center for education reform has taken a strong view on the subject of choice with charter schools. They believe that if the students choose to attend a certain school and the teachers choose to teach at a certain school, then the chances of success are more likely because both have chosen to be there (Center for education reform, 1999).

After Twenty Years Essay

â€Å"After Twenty Years† by O. Henry, is a great story. It is about two men who are great friends; they schedule a meeting so they can meet after twenty years, hence the title of the story. This story illustrates that people change but that does not necessarily mean in a good way! I had many friends when i changed schools and none of the friends i had changed in a good way, they now smoke and dress inappropriately. â€Å"After Twenty Years† really grabs the reader’s attention and O. Henry made a surprise ending that caused the story to end with a BANG! it truly was an amazing story. Bob and Jimmy were great friends and scheduled an appointment to meet after twenty years in front of an old restaurant. BY the time the day comes, the restaurant is now a shop. Bob was waiting in front of the locked doors that night when a cop approaches him and right away, Bob explains why he is there to assure the cop its okay for him to be there. To me, it seems a little suspicious to explain right away. The cop listens and planned to stay. Then he lights a cigar and the cop got glimpse of his face. After hearing the story he walks away. Twenty minutes later, JImmy shows up. They go walking and talk about how Bob is wealthy and that he lives out West. Jimmy listens for a bout ten minutes then Bob notices his nose. â€Å"Twenty years is a long time but not long enough to change a Roman into a Pug.† He explains concerned. Then the under cover cop explains and hands him a note. The note said, Bob, I was at the appointed place on time. when you lit the cigar i saw the face of a man wanted in Chicago, i knew i couldn’t arrest you myself so i sent and under cover officer, Jimmy. The ending really surprised me and it was a good story. My friends may have never robbed anything from the West but they were bad. Do you have anyone who changed? Perhaps yourself?

Books Will Never be Replaced Essay examples - 1692 Words

Books Will Never be Replaced What is it about a book, the physical book, the tangible reincarnation of an author that allows us to believe it will continue to be revered and regarded even in our age of computerized information? How can we be sure that, just as the clay tablet gave way to the scroll and bound book, the faith we have placed in paper editions will not be improved upon with the microchip? It may be that for all our attempts to squeeze and shrink information into screens, to encapsulate a world of knowledge into the size of a suitcase, to create a communications device which is always targeted at fitting into the palm of ones hand, there exists a hand-held favorite already which has worked itself inextricably into the†¦show more content†¦Ovid is not the only one. For centuries, authors have trusted that paper life is somehow timeless, a monument more lasting than bronze of which another Roman poet, Horace, spoke. It is strange that we invest such faith in paper. Paper is one of the thinnest vessels of trust, to be sure, for all its fragility and sensitivity to time. The very mention of library fire at Alexandria makes us nervous about the transient nature of all knowledge. It is the Dream of the Arab in Wordsworths Prelude, a nightmare that some catastrophic flood or holocaust might wipe out all collected human understanding which must lodge in shrines so frail. And indeed, though many of Ovids book-sons have come down to us, one of his daughters, the tragedy Medea, remains lost. So why do we continue to put our faith in books? Certainly, one answer is that through replication and distribution we might avoid the realization of Wordsworths nightmare. Paper, we have always believed, is a renewable resource, and the libraries of any country provide a tacit vigilance over the treasures of human finding. Is the thought of a computerized library any less worrisome, since computers require a great deal more energy and expertise to keep in order? Imagine the irony of some cataclysmic worldwide computer crash or power failure wherein the store of human knowledge, our only refuge against the sands of time, would be forever wedged in silicon. Paper,Show MoreRelatedThe Civil War And Reconstruction977 Words   |  4 PagesA revolution is a dramatic and sudden change in an organization in the social order that is replaced by a new, more favorable system. When Historian Eric Foner called the Reconstruction period â€Å"America’s Second Revolution†, his charac terization was correct. Reconstruction can be viewed as a revolution because the previous social order, slavery, was replaced suddenly by a more favorable one, freedom for African-Americans. There was a long period of politicization for incorporating free African-AmericansRead MoreE-Books vs. Paperback Books1583 Words   |  7 PagesE-BOOKS VS. PAPERBACK BOOKS Recently, e-book came out and developed rapidly. In 2012, Amazon released news that it sold more e-books than paperback books in the global market in 2011. Interestingly, it was the first time more e-books than paperbacks were sold on Amazon. Some people have predicted that one day e-books will completely replace paperback books. They saw the rapid increase of e-books’ selling and potential. Undeniably, today’s society has a tendency to prefer e-books. But this preferenceRead More Books of the Future1683 Words   |  7 Pages For hundreds of years people have been documenting their works through bounded pages known as books. Whether these accounts are fiction or non-fiction, the stories and experiences authors tell have been enjoyed by millions across the globe. While readers take pleasure in cuddling up with an intriguing book and forgetting all of the troubles of life, they can dive into the adventures of a person other than themselves. Reading can take a person i nto times of war and famine, to far-away lands ofRead MoreRay Bradbury s Fahrenheit 4511721 Words   |  7 Pagesattempts to answer. In this book, he describes a hypothetical world in which the population not only avoids reading, but has made owning books an unthinkable crime, with all books discovered burned, along with the houses of those who hoarded them. In this dystopian future created by Bradbury, the beauty that is literature has been replaced in society by television programs and radio stations. This reveals Bradbury’s obvious fear that modern technology would completely replace books- a legitimate concernRead MoreTomb of Tausert and Setnakht1044 Words   |  5 Pagesburial. Apparently his son, Ramesses III, went against his father’s wishes and decided to bury him in the tomb of Tausert. It was then that KV 14 was remodeled as Setnakht’s burial tomb. The scenes of Tausert on the walls were plastered over and replaced with figures and names of Setnakh t. The plaster has fallen away in some parts of the tomb, exposing parts of the earlier scenes that were originally there. Throughout the tomb’s first three corridors, there are holes cut into the walls. These holesRead MoreArtificial Intelligence Is The Most Controversial Field877 Words   |  4 PagesWithin the last 50 years, the information scientists know about artificial intelligence has increased to levels never reached before. In 1968, the first computer controlled walking machine was created and today, robots are replacing humans in the work industry (History 5). By the year 2029, it is predicted that robots will be able to outsmart their makers (Khomami 1). More knowledge about artificial intelligence is being acquired by scientists. There are several advantages that come with artificialRead MoreThe Adventures Of Huckleberry Finn By Mark Twain983 Words   |  4 Pagesof this there has been a controversy about whether the n-word should be removed and replaced with t he term â€Å"slave† or whether it should be left the way it already is. Although Twain intended for the word to be in the novel, because he wanted to give a vivid experience of what it felt like to live in the South during the 1830’s. But should there really be a version of the book with the n-word removed and be replaced with the term â€Å"slave,† even though we’ll be destroying Twain’s purpose of the novelRead MoreManagement1102 Words   |  5 Pagesthe art of getting things done through and with people in formally organised groups. Harold Koontz gave this definition of management in his book The Management Theory Jungle. According to Henri Fayol, To manage is to forecast and to plan, to organise, to command, to co-ordinate and to control. Henri Fayol gave this definition of management in his book Industrial and General Administration. Image Credits  © Michael Heiss. According to Peter Drucker, Management is a multi-purpose organRead MoreEssay on Failed Revolutions and Tyrants in Animal Farm649 Words   |  3 PagesAnimal Farm. The animals are swayed by his propaganda and are fearful of his police dogs; this is what keeps him in power. Napoleon’s disastrous reign only supports Orwell’s idea that revolutions always fail, tyrants are only replaced, and a new government is never established. Napoleon is a cruel ruler who, fearful of Snowball’s return, executes all the animals who â€Å"confess† to being in league with Snowball. Napoleon uses Squealer as a propaganda spreader to the other animals of the farm. Squealer